Figure 8.

Inversions induced by HR between Ds elements. (A) Genomic structures of parent and inversion lines are shown. Numbers indicate positions of Ds-y1, Ds-y2 and EcoRI sites in chromosome 1. Other symbols as in Figure 3. (B) Southern blot analysis of putative HR-induced inversion lines. Genomic DNA from the indicated lines was digested with EcoRI and hybridized with probe 5A. Lines D4 and D35 showed bands of the same size as the wild type allele (5.7 kb), consistent with excision of both Ds-y1 and Ds-y2. Lines D7 and D64 showed bands of the same size (5.3 kb) as parental line (OsRLG5-161), consistent with retention of both Ds-y1 and Ds-y2. Line D5 contained one parental band (5.3 kb) and one shorter band (4.7 kb), suggesting heterozygosity for a parental allele (both Ds elements retained) and the D5 allele in which Ds-y2 had excised. W, wild-type; P, parental line.