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. 2011 Oct 8;39(22):e155. doi: 10.1093/nar/gkr829

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — (A) Proposed mechanism of the theophylline-responsive TetR aptamer. In presence of theophylline the TetR aptamer structure is restored. This allows for specific binding to tTA thus inhibiting transcription initiation. P_hCMVmin*−1 stands for tetracycline responsive promoter, SEAP for secreted alkaline phosphatase and pA for poly(A)-tail. (B) Plasmid constructs used in this work. pDAX encodes either pDA1 (active TetR aptamer), pDA3 (inactive TetR aptamer) or pDA4 (theophylline-responsive TetR aptamer). pSAM200 encodes tTA, pMF111 contains a P_hCMV*−1-driven SEAP expression unit.