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. 2011 Oct 8;39(22):e155. doi: 10.1093/nar/gkr829

Figure 2.

Figure 2.

The TetR aptamer in mammalian cells. (A) Nucleotide sequence and secondary structure of the TetR aptamer variant 12-1R adapted from Hunsicker and colleagues (33). Boxed nucleotide indicates an inactivating point mutation (33). (B) Transcription of the TetR aptamer in HEK293-T cells significantly represses reporter gene expression compared to the genetically inactivated control variant. (C) Analysis of RNA expression of different TetR aptamer constructs in HEK293-T cells by semi-quantitative RT–PCR showed comparable expression levels of all constructs.