Skip to main content
. 2011 Aug 31;39(22):9633–9648. doi: 10.1093/nar/gkr682

Figure 1.

Figure 1.

FBP1 associates with EV71 5′-UTR. (A) FBP1 association with EV71 5′-UTR was confirmed by competition assay and western blot. The biotinylated RNA association proteins were loaded to SDS–PAGE (12%). FBP1 antibody was utilized in this western blot. Various amounts of unlabeled EV71 5′-UTR and yeast tRNA RNA probe were added to compete with the biotinylated EV71 5′-UTR probe interacting with FBP1 in RD cell lysate. Lanes 1 and 6 contained cell lysate (200 µg) only. An unlabeled EV71 5′-UTR RNA probe was used in the competition assay (lanes 3–5), and an unlableled yeast tRNA probe was utilized (lanes 8–10). (B) EV71 5′-UTR associates with cellular protein FBP1 in the various cell lines, SK-N-MC, SF268, RD and Vero cell. Cell lysates are shown in lanes 1, 6, 11 and 16. Various cell extracts were incubated in the absence of RNA (lanes 2, 7, 12 and 17) or in the presence of biotin-16-UTP only (lanes 3, 8, 13 and 18), non-biotinylated EV71 5′-UTR (lanes 4, 9, 14 and 19) or biotinylated EV71 5′-UTR (lanes 5, 10, 15 and 20). After the streptavidin beads were washed, the EV71 5′-UTR associated proteins were detected using SDS–PAGE (12%). FBP1 protein was analyzed by western blot with anti-FBP1 cellular protein antibody.