Figure 7.

Chromatin remodeling precedes the increase in transcription factors recruitment to the BLV 5′-LTR in YR2 cells. Chromatin prepared from untreated or PMA/ionomycin-treated YR2 cells (for 15, 30, 60, 90 and 120 min) was immunoprecipitated with specific antibodies directed against chromatin marks or chromatin modification enzymes (A), transcription factors and RNA polymerase II (B) or with an IgG antibody as a control. Purified DNA was then amplified with oligonucleotide primers hybridizing in the BLV 5′-LTR (upper panel for each antibody) or in an unrelated region corresponding to the BLV env gene (down panel for each antibody). Results are presented as histograms indicating percentages of immunoprecipitated DNA/input DNA (% IP/In). The first bar corresponds to IgG control and the second bar to the indicated specific antibody. Data are the mean ± SEM of triplicate samples from one representative of three independent experiments.