Figure 2.

Large cargos delivered by giant PB transposons are intact. (a) Precise integration of giant PB transposons at the expected TTAA site. The chromosomal coordinates of the first T corresponding to the PB recognition site TTAA are shown (NCBI m37). The shaded sequences represent the ends of the PB ITRs. (b) Southern blot illustrating the copy number of the PB mediated largo-cargo integrations using the PB5′ITR as the detection probe. (c) Regional CGH analysis showing the gain of an extra copy of the human HPRT gene delivered by PB transposition. The red line was calculated as the running median of the Log₂ value of each CGH probe to aid the visualization.