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. 2011 Sep 24;39(22):e148. doi: 10.1093/nar/gkr764

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Stable integration of a PB transposon harboring the human FAH locus. (a) Schematic representation of the PB-FAH-62.5 construct and the selection strategy used for enriching genuine transposition events. (b) Transposition efficiency of PB-FAH-62.5 using different transfection methods and selection schemes. ‘Colonies analyzed’: the number of colonies analyzed by splinkerrette PCR for the determination of the PB ITR to genomic junction sequence. Asterisk indicates percentage of transposition events as a fraction of colonies analyzed.