Table 1.

Predicted ΔG° values of each tRNA anticodon class

tRNA anticodon class	Number of tRNAs	Number of 3-bp combinations	Measured ΔG° Phe-tRNAX (kcal/mol)d	Mean Calculated ΔG° Phe-tRNAX (kcal/mol)	σ	R
Ala CGC	58	20		−10.3	0.50	0.999
Ala GGC	92	23	−11.0	−10.0	0.53	0.996
Ala UGC	93	25		−10.0	0.52	0.998
Arg ACGa	149	31	−9.6	−9.4	0.32	0.990
Arg CCGa	161	45		−9.5	0.58	0.974
Arg CCU	207	51		−9.6	0.59	0.992
Arg UCU	226	41		−9.3	0.49	0.983
Arg UCGa	66	23		−9.4	0.32	0.987
Asn GUU	160	23	−9.4	−9.4	0.60	0.998
Asp GUC	75	16	−11.5	−11.0	0.33	0.988
Cys GCA	175	30	−10.1	−9.9	0.62	0.992
Gln CUG	83	32	−8.8	−8.9	0.50	0.984
Gln UUG	88	32		−9.1	0.56	0.997
Glu CUC	70	17		−10.3	0.50	0.998
Glu UUC	117	21	−12.2	−10.4	0.48	0.997
Gly CCC	78	10		−11.0	0.34	0.991
Gly GCC	76	13	−11.2	−11.0	0.39	0.980
Gly UCC	99	19		−10.8	0.68	0.981
His GUG	180	22		−10.7	0.56	0.997
Ile GAU	93	34	−9.2	−9.4	0.36	0.998
Leu CAA	237	26		−10.0	0.50	0.998
Leu CAG	146	22	−10.0	−9.9	0.51	0.981
Leu GAG	152	23		−10.4	0.60	0.989
Leu UAA	232	26		−10.3	0.43	1.000
Leu UAG	194	20		−10.2	0.49	0.941
Lys CUU	117	30		−9.4	0.86	0.989
Lys UUU	151	35	−10.0	−9.7	0.76	0.980
Met/fMet/Ile CAU	320	69		−9.5	0.94	0.970
Met CAUb	89	34	−9.9	−9.9	0.85	0.926
fMet CAUb	43	24	−8.9	−9.1	0.55	0.973
Ile CAUb	101	31		−9.3	0.48	0.984
Phe GAAa	118	34	−9.9	−9.4	0.82	0.943
Pro CGG	83	20		−9.8	0.36	0.993
Pro GGG	86	24		−9.8	0.56	0.995
Pro UGGa	141	25	−9.9	−9.6	0.54	0.994
Sec/Trp UCA	63	36		−8.2	1.2	0.706
Sec UCAc	56	15	−8.0	−8.2	1.2	0.706
Trp UCAc	7	6		−9.6	0.75	0.895
Ser CGA	162	20		−10.2	0.35	0.993
Ser GCU	176	20		−10.2	0.37	1.000
Ser GGA	170	17		−10.3	0.32	1.000
Ser UGAa	218	20	−9.6	−10.1	0.32	0.923
Thr CGU	148	48	−11.0	−10.0	0.67	0.995
Thr GGU	158	41		−10.1	0.52	0.999
Thr UGU	167	53		−9.8	0.57	0.982
Trp CCAa	154	37	−9.0	−9.4	0.40	0.995
Tyr GUA	139	31	−8.6	−9.2	0.38	0.997
Val CAC	64	25		−9.5	0.45	0.996
Val GAC	137	33		−9.7	0.41	0.998
Val UACa	141	28	−9.3	−9.4	0.49	0.925

^aUses experimentally derived value for tRNAs containing G49C65 G50U64 G51C63.

^btRNAs curated in (20).

^ctRNAs curated using tFAM 1.3 (21).

^dData from (8).