Table 2.

ΔΔG° values of single base pair mutations in three tRNAs

	tRNAPhe ΔΔG° a (kcal/mol)	tRNALeu ΔΔG° ab (kcal/mol)	tRNAThr ΔΔG° c (kcal/mol)	(kcal/mol)
49–65 CG	0.0	0.0	0.0	0.0
49–65 AU	−0.5	−0.3	−0.8	−0.5
49–65 GC	−0.4	−0.2	−0.7	−0.4
49–65 UA	−0.1	N.D.	−0.2	−0.2
49–65 GU	−0.8	−0.9	−1.1	−0.9
50–64 UA	0.0	0.0	N.D.	0.0
50–64 AU	−0.1	0.1	N.D.	0.0
50–64 GC	−0.2	0.3	N.D.	0.0
50–64 CG	−0.5	0.1	N.D.	−0.2
50–64 GU	1.3	1.4	N.D.	1.4
50–64 UG	0.3	0.5	N.D.	0.4
51–63 GC	0.0	0.0	0.0	0.0
51–63 AU	0.8	1.4	1.0	1.1
51–63 CG	0.5	0.6	0.4	0.5
51–63 UA	0.9	1.0	1.2	1.0
51–63 GU	1.0	1.1	0.2	0.8
51–63 UG	1.0	N.D.	N.D.	1.0
51–63 AC	0.2	N.D.	N.D.	0.2

ΔΔG° values are calculated relative to the sequence of tRNA^Phe designated in bold.

^aData from (15).

^bData from (14,15).

^cData from (25).