Table 4.

tRNAs with anomalous calculated ΔG° values

tRNA	Species	Mean Isoacceptor ΔG° (kcal/mol)	Calculated ΔG° (kcal/mol)	tRNA Chimera ΔG° (kcal/mol)	tRNAPhe Chimera ΔG° (kcal/mol)
Another gene copy present
Ala CGC	Sorangium cellulosum	−10.3	−9.0	−9.3 ± 0.6	−8.9 ± 0.6
Arg ACG	Sorangium cellulosum	−9.2	−10.7
Arg ACG	Pseudomonas aeruginosa	−9.2	−10.7
Asn GUU	Bradyrhizobium japonicum	−9.4	−10.7
Gln UUG	Mesorhizobium loti	−9.1	−7.7
Glu UUC	Thiomicrospira denitrificans ATCC 33890	−10.4	−8.1
Glu UUC	Helicobacter pylori	−10.4	−8.0	−8.6 ± 0.04	−8.4 ± 0.1
Gly GCC	Acaryochloris marina MBIC11018	−11.0	−9.4
Gly UCC	Staphylococcus species	−10.8	−9.5	−9.6 ± 0.3	−9.7 ± 0.2
Ile GAU	Mycobacterium vanbaalenii PYR-2	−9.4	−10.7
Ile GAU	Leuconostoc mesenteroides ATCC 8294	−9.4	−8.1
Thr CGU	Bifidobacterium longum	−10.0	−8.1	−8.3 ± 0.5	−8.3 ± 0.3
Only gene copy present
Arg ACG	Aquifex aeolicus	−9.2	−10.7
Arg CCG	Geobacter sp FRC32	−9.5	−11.0
Arg CCG	Candidatus Ruthia magnifica Cm Calyptogena magnifica	−9.5	−11.0
Arg CCG	Candidatus Vesicomyosocius okutanii HA	−9.5	−11.0
Arg CCG	Bifidobacterium longum	−9.5	−11.0
Arg CCU	Rubrobacter xylanophilus	−9.6	−8.0	−9.1 ± 0.4	−8.4 ± 0.1
Arg CCU	Fusobacterium nucleatum	−9.6	−11.0	−10.8 ± 0.6	−10.9a
Arg UCU	Clavibacter michiganensis	−9.3	−10.7
Arg UCU	Lactobacillus sakei	−9.3	−10.7
Arg UCU	Zymomonas mobilis	−9.3	−10.7
Arg UCU	Acidiphilium cryptum JF-5	−9.3	−10.7
Arg UCU	Chloroflexus aurantiacus J 10 fl	−9.3	−10.7
Arg UCU	Opitutus terrae PB90	−9.3	−10.7
Arg UCU	Renibacterium salmoninarum ATCC 33209	−9.3	−10.7
Arg UCU	Lactobacillus reuteri F275	−9.3	−10.7
Arg UCU	Bacteroides fragilis NCTC 9434	−9.3	−10.6
Arg UCU	Cytophaga hutchinsonii ATCC 33406	−9.3	−10.6
Asn GUU	Borrelia afzelii Pko	−9.4	−8.0
Gln CUG	Lactobacillus acidophilus NCFM	−8.9	−10.3
Gln UUG	Solibacter usitatus Ellin6077	−9.1	−7.7
Gln UUG	Candidatus Pelagibacter ubique HTCC1063	−9.1	−7.6
Leu GAG	Bradyrhizobium japonicum	−10.4	−9.1	−9.8 ± 0.6	−9.4a
Lys CUU	Ehrlichia ruminantium Gardel	−9.5	−11.0
Pro CGG	Lactobacillus acidophilus NCFM	−9.9	−11.2
Thr GGU	Ehrlichia canis Jake	−10.1	−8.6
Thr GGU	Mycoplasma genitalium	−10.1	−8.2
Thr GGU	Mycoplasma gallisepticum	−10.1	−8.2
Thr UGU	Corynebacterium urealyticum DSM 7110	−9.8	−11.2	−10.5 ± 0.3	−11.2a
Thr UGU	Mesorhizobium loti	−9.8	−8.6	−10.0 ± 0.7	−8.7 ± 0.4
Thr UGU	Candidatus Pelagibacter ubique HTCC1063	−9.8	−8.2
Thr UGU	Dinoroseobacter shibae	−9.8	−8.2
Tyr GUA	Borrelia afzelii Pko	−9.2	−10.5

Both calculated and measured ΔG° are for phenylalanylated tRNAs.

^aΔG° measured in (15).