Table 2.
Fold change in mRNA levels in the Sox6 KO skeletal muscles compared to control
| TA | EDL | Gas | Sol | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mouse ID# | 1 | 2 | 3 | 1 | 2 | 3 | 1 | 2 | 3 | 1 | 2 | 3 |
| Sox6 | 0.75 | 0.42 | 0.09 | 0.43 | 0.38 | 0.07 | 0.38 | 0.53 | 0.10 | 0.20 | 0.31 | 0.12 |
| Myh1 (IIx/d) | 2.08 | 1.68 | 0.20 | 1.31 | 5.77 | 5.49 | 6.79 | 14.80 | 2.11 | 0.01 | 0.03 | 1 × 10-3 |
| Myh2 (IIa) | 5.00 | 1.96 | 0.80 | 3.08 | 2.11 | 4.72 | 6.63 | 5.77 | 1.85 | 0.02 | 0.02 | u.d. |
| Myh4 (IIb) | 0.17 | 0.01 | 0.01 | 0.11 | 0.04 | 0.02 | 0.25 | 1 × 10-3 | 0.02 | 0.16 | 0.16 | 0.01 |
| Myh6 (α) | 78.79 | 252.99 | 1.03 | 24.19 | 25.37 | 6.65 | 3.54 | 4.13 | 2.42 | 4.03 | 1.61 | 0.65 |
| Myh7 (β) | 9042.52 | 2177.81 | 94.12 | 1318.30 | 728.63 | 1611.29 | 21.86 | 3.59 | 9.68 | 3.60 | 1.00 | 1.20 |
| Myh7b | 31.37 | n.d. | 0.49 | 3.98 | n.d. | 1.39 | 14.30 | n.d. | 3.06 | 1.51 | n.d. | 0.34 |
| Myl2 | 1496.57 | n.d. | 7.07 | 57.22 | n.d. | 55.59 | 7.37 | n.d. | 2.70 | 1.91 | n.d. | 1.31 |
| Tnnc1 | 6830.19 | 4622.47 | 474.50 | 665.43 | 687.83 | 128.70 | 91.24 | 167.58 | 14.52 | 0.81 | 2.98 | 1.53 |
| Tnni1 | 2619.20 | 1867.14 | 610.53 | 433.38 | 552.33 | 861.06 | 116.58 | 69.76 | 24.71 | 2.64 | 2.58 | 2.59 |
| Tnni2 | 0.34 | 0.50 | 0.20 | 0.36 | 0.89 | 0.15 | 0.46 | 0.76 | 0.35 | 0.01 | 0.14 | 2 × 10-3 |
| Tnnt1 | 4348.43 | 1594.44 | 157.77 | 1011.39 | 510.51 | 153.18 | 131.98 | 59.18 | 5.07 | 2.93 | 1.76 | 1.56 |
| Tnnt2 | 2.45 | 1.88 | 2.08 | 2.31 | 0.59 | 1.55 | 1.23 | 2.65 | 1.37 | 2.59 | 1.12 | 1.85 |
| Tnnt3 | 0.45 | 0.40 | 0.43 | 0.28 | 0.89 | 0.58 | 0.34 | 0.38 | 0.19 | 4 × 10-3 | 0.06 | 2 × 10-4 |
| Chrng (fetal) | 0.89 | 4.63 | 4.94 | 0.73 | 1.80 | 1.82 | 1.23 | 1.80 | 3.29 | 2.78 | 1.17 | 8.26 |
| Chrne (adult) | 0.44 | 0.30 | 0.28 | 0.14 | 0.18 | 0.76 | 0.16 | 0.15 | 0.14 | 0.24 | 0.34 | 1.44 |
| Prox1 | 29.26 | 10.30 | 9.66 | 22.84 | 6.68 | 17.04 | 16.21 | 8.41 | 3.34 | 3.79 | 2.26 | 1.27 |
| Tead1 | 1.83 | n.d. | 0.99 | 0.63 | n.d. | 0.98 | 1.25 | n.d. | 0.78 | 1.02 | n.d. | 0.94 |
| Tead4 | 3.03 | n.d. | 1.26 | 1.34 | n.d. | 1.08 | 1.23 | n.d. | 1.08 | 0.96 | n.d. | 1.11 |
| Tcf4 | 1.94 | n.d. | 1.70 | 0.88 | n.d. | 1.54 | 1.68 | n.d. | 1.15 | 0.88 | n.d. | 1.43 |
| Hdac9 | 4.44 | n.d. | 2.68 | 1.51 | n.d. | 1.79 | 7.56 | n.d. | 1.42 | 1.34 | n.d. | 0.75 |
| Myod1 | 0.73 | 1.01 | 0.70 | 0.73 | 1.02 | 2.11 | 1.47 | 1.44 | 1.17 | 1.51 | 1.77 | 0.72 |
| Myog | 3.64 | 1.52 | 2.24 | 1.98 | 1.84 | 3.00 | 2.61 | 1.12 | 0.95 | 4.48 | 1.89 | 1.35 |
| Mb | 1.84 | 1.47 | 1.48 | 0.66 | 2.30 | 1.75 | 1.88 | 3.50 | 1.54 | 0.77 | 1.62 | 1.09 |
| Sdha | 1.18 | 0.74 | 0.39 | 0.70 | 0.74 | 0.50 | 0.79 | 0.60 | 0.32 | 0.68 | 0.59 | 0.55 |
| Ppargc1a | 1.28 | 1.39 | 0.53 | 0.82 | 0.78 | 0.35 | 0.63 | 1.46 | 0.42 | 0.53 | 0.71 | 0.19 |
Sox6 was inactivated using MCK-Cre mice. Mouse 1 and mouse 2 are two month-old, and mouse 3 is three month-old. Gene names in bold: associated with Sox6 binding. Gene names in italics: slow fiber specific sarcomere proteins, or transcription factors reported that are preferentially expressed in slow fibers. n.d.: not determined. u.d.: undetected in Sox6 KO mouse. Control expression level = 1.00.