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Western blots show sumoylation of recombinant human topo I. Recombinant human topo I protein was subjected to the sumoylation reaction and examined by Western blotting using anti-topo I (I) and anti-SUMO1 antibodies (II). Compared to topo I protein without sumoylation reaction (topo I A), topo I protein with sumoylation reaction (topo I B) showed poly-sumoylation of topo I (II). The assays showed similar results in triplicates.
