Figure 8.

NPY decreases FcγRIIA-myc expression on microglial cells. (A) Densitometric quantification of western blots shows that microglial cells treated with LPS (100 ng/ml) or with IL-1β (1.5 ng/ml) increased FcγRIIA expression levels while NPY treatment (1 μM) significantly inhibited this effect. (B) Representative confocal photomicrographs demonstrate an inhibitory effect of NPY on LPS- or IL-1β-induced FcγRIIA expression. Cells were stained for FcγRIIA-myc (in red), CD11b (in green) and Hoechst 33342 (nuclei in blue). Data are expressed as mean ± SEM (n = 4) and as a percentage of control (*p < 0.05, using Bonferroni's Multiple Comparison Test). Scale bar 10 μm.