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Y2H direct interaction assays. The Brachypodium NF-YC orthologs Bradi1g32200, Bradi1g67980, and Bradi3g05270 were cloned as Gal4 DNA binding domain (DBD) fusions and directly tested for the ability to physically interact with previously described Gal4 activation domain (AD) fusions to full length Arabidopsis CO, TOC1, NF-YB2, and NF-YB3 [28]. EV - empty vector; MC - manufacturer's (Invitrogen) controls (+ strong interactor, +/- weak interactor, - non-interactor).
