Figure 3. Different protein isoforms of Tra2β can act as specific splicing activators, co-activators, and repressors of a target exons identified by HITS-CLIP.

(A) Efficient protein expression levels of different GFP fusion proteins used in these experiments (upper panel). Levels of actin were measured in parallel (lower panel). (B)–(J). Upper panels: Bar charts showing percentage splicing inclusion (PSI) of a panel of exons identified through HITS-CLIP in response to GFP and Tra2β-GFP fusion proteins. All data used to make the bar charts was from at least 3 biological replicates, and the error bars are shown as standard errors. Lower panels: Representative capillary gel electrophoresis image from each RT-PCR analysis. Probability (p) values were calculated using an independent two-sample T-test between the PSI levels for cells co-transfected with GFP and each of the different Tra2β-GFP constructs (* p≤0.05, **p≤0.01).