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. 2011 Dec 15;7(12):e1002394. doi: 10.1371/journal.pgen.1002394

Figure 1. HIF-1 is required for ftn-1 and ftn-2 transcriptional repression during iron deficiency.

Figure 1

(A) Conserved sequences in the 5′ promoter regions of ftn-1 and ftn-2. Large stripped boxes, IDE; small boxes, GATA binding motifs. The IDE is enlarged showing HRE1, HRE2 and HRE3 and GATA1 and GATA2 sequences. Arrows indicate orientation of motifs. (B and C) Expression of ftn-1::GFP-his and ftn-2::GFP-his reporters in L4 larvae fed control (empty vector L4440) RNAi or hif-1 RNAi after culture on NGM or NGM-BP for 16 h. Quantification of GFP expression in (B and C) was carried out using the COPAS Biosort. Dot plots of sorted worms are shown where each dot represents one worm. Data are mean GFP fluorescence ± SEM reported as a percentage of worms fed control RNAi on NGM (n = 1000 worms per treatment), *p<0.0001.