Skip to main content
. 2011 Dec 15;7(12):e1002414. doi: 10.1371/journal.pgen.1002414

Figure 6. The stem length and sequence are important but can tolerate minor modifications.

Figure 6

(A) Modifications in the stem of the hairpin/cruciform structure are shown. StD2, StD4 and StD6 are deletion mutants; StI2, StI4 and StI8 are insertion mutants. StAT has four GC base pairs in the middle of the stem changed to AT base pairs. Boxed TAG, mtd stop codon. (B) The stem is important for BPP-1 DGR mutagenic homing but can tolerate minor changes. PCR-homing assays are shown along with primers used. (C) Effects of stem modifications on phage tropism switching frequencies. The scale bars represent mean TSF ± s.d. P values comparing mutants to WT in Student's t tests are indicated with asterisks. *P<0.02.