Figure 1. The eukaryotic translation initiation factor eIF4G3 is degraded by GrB (1 µg/ml) in Jurkat (Jkt) cells.

(A) Representative Western blot analysis of detectable full-length eIF4G3 and Bid activation in Jurkat cells from time 0 (no GrB treatment) to 30 min post GrB/AD treatment (left panel) and in the presence of zVAD-fmk (right panel). (B) Densitometric analysis of eIF4G3 (A) where the eIF4G3/α-tubulin ratio was calculated and the highest densitometric value was 100%. Other values were plotted as a percentage of the maximum densitometric value. Statistical significance: p<0.01 (**); (n = 3 of 3 independent experiments).