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. 2011 Dec 15;6(12):e26274. doi: 10.1371/journal.pone.0026274

Figure 3. CCL22 secretion induced from the RAW 264.7 macrophage cell line.

Figure 3

(A) RAW264.7 cells were plated on 96 well U-bottom tissue culture plates at 5×105 cells per well and incubated in culture medium (10% FBS/DMEM with 1% PSG) without or with molar equivalent concentrations of copolymers for 24 hours at 37°C with 5% CO2 in triplicate. Cell-free culture supernatant was collected and immediately frozen at −80°C for testing using a commercial CCL22 ELISA kit. Data are represented as a non-linear regression curve fit and shown as mean (pg/mL) ± SEM. (B) Linear correlation be-tween area under the curve of CCL22 production and the length/molecular weight (Da) in the culture supernatants of RAW264.7 cells incubated in culture medium with YEAK, YFAK, or molar equivalent of truncated YFAK (r2 = 0.9637, p<0.0001.