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. 2011 Dec 15;6(12):e28020. doi: 10.1371/journal.pone.0028020

Figure 2. In vitro transcription assays show dominant negative activity.

Figure 2

A Cos-7 cell transfection assay described in the Materials and Methods was used to measure the ability of Ahr2 and ARNT to induce luciferase reporter expression when different Ahr2 mutants were co-expressed in the system. Light bars indicate activity in the absence of TCDD; dark bars indicate activity in the presence of TCDD (1 ng/ml). Units are Relative Luciferase Units, a dimensionless ratio of luciferase activity from the luminometer divided by the β-galactosidase activity from the control reporter used for transformation normalization [5], [26]. Single asterisks: different from control no TCDD value, p<0.05. Double asterisk significantly different from control +TCDD value, p<0.05.