Figure 3. Western blot confirms expression of Ahr2^1–440 in transgenic zebrafish lines.

Embryos from the transgenic lines 15, 26 and 31, carrying the Ahr2^1–440 transgene were processed for western blotting with an anti-myc antibody as described in Materials and Methods. The lane carrying the mutant Ahr2^1–440 protein made in vitro for use as a positive control is indicated as TNT, and a negative control lane loaded with extract from wild type embryos having no myc tag is indicated as WT.