Figure 2. Maps of plasmids pJA1, pJA2 and pJA3 after linearization in the cut cassette.

The 1 kb 5′ part of lacZ is shown with a grey box, and the 1 kb 3′ part of lacZ (lacZend) by a white arrow. The cat gene encoding resistance to chloramphenicol (Cm^R) is shown as a stripped arrow. The cut cassette is indicated by a small black box, linearization of pJA plasmids in vivo might happen either at the IsceI recognition sequence, or between the two EcoK sites. The cut cassette map is shown in details, with two grey boxes indicating the Chi sites. Below the three plasmids, the E. coli chromosome with which plasmids might recombine is shown (wavy line) in the lacZ region (grey to white arrow).