Table 5. Recombination frequencies with linear pJA2 in E. coli mutant derivatives.
MAC1306 Donor×Recipient strain | Relevant genotype of recipient | Nb of expe-riments | Av. LacZ+CmR/recipient | Fold, relative to WT(1) | Frequency LacZ −CmR/recipient(2) | Fold, relative to WT | Proportion of HFIR events among LacZ− |
JAC7 | WT | 31 | 4.2 (+/−3.4)×10−6 | 1 | 4.1×10−9 | 1 | 4/60 |
MAC1348 | recA | 12 | 1.3 (+/−0.8)×10−8 | 0.003 ** | <8.7×10−10 | <0.21 | NA |
MAC1397 | recB | 12 | 7.0 (+/−7.0)×10−7 | 0.17 ** | <2.1×10−9 | <0.26 | NA |
MAC1473 | recF | 12 | 1.1 (+/−0.6)×10−6 | 0.26 ** | 1.8×10−9 | 0.31 | 1/9 |
MAC1357 | ruvA | 12 | 2.5 (+/−2.8)×10−5 | 5.8 * | 1.1×10−8 | 1.90 | 0/9 |
MAC1479 | recG | 12 | 7.5 (+/−6.3)×10−7 | 0.18 ** | 1.5×10−9 | 0.26 | 0/2 |
MAC1352 | recD | 15 | 1.8 (+/−1.0)×10−5 | 4.2 ** | 4.4×10−10 | 0.07 | 0/3 |
MAC1350 | recJ | 12 | 3.7 (+/−2.6)×10−6 | 0.87 | 6.2×10−9 | 1.51 | 0/22 |
*Significant below 5%, ** Significant below1% with a Student test.
Frequencies calculated with the first term of a Poisson law.