Figure 4. Effect of an acute treatment with galactose medium on myotube aerobic capacity.

A. Basal oxygen consumption rate. B. State 4 respiration (leak dependent; non-phosphorylating). After basal oxygen consumption rate measurement, cells were treated with oligomycin (600 ng/ml) to determine state 4 respiration. C. Maximal oxygen consumption capacity. After basal and state 4 respiration, cells were treated with FCCP (1 µM) to determine maximal oxygen consumption. A–C. Myotubes were differentiated for 7 days in LG (5 mM glucose). On the 7^th day of differentiation, myotubes were exposed to HG (25 mM), LG (5 mM glucose) or GAL (10 mM galactose) for 45 min before measuring basal oxygen consumption rate. Results are presented as means ± SEM, n = 4, in which each condition was assessed in 5 replicates.