Skip to main content
. 2011 Dec 15;6(12):e29173. doi: 10.1371/journal.pone.0029173

Figure 2. Reciprocal expression of IGF-1R and miR-133 during muscle development and C2C12 cell differentiation.

Figure 2

(A) IGF-1R protein and miR-133 levels were determined in hind limb muscles at 18.5 dpc embryos, 2 days postnatal neonates or adults in C57BL/6J mice by western blot or northern blot analysis. GAPDH or U6 snRNA served as loading controls for protein or small RNA. (B) Morphology of proliferating myoblasts maintained in growth medium or differentiated myotubes after serum derivation for 6 days. (C) C2C12 myoblasts were induced to differentiate for up to 8 days. Protein levels were determined by western blotting, miRNA by northern blotting and mRNA by semi-quantitative RT-PCR. Representative results from independent experiments (n≥2) are shown. The numbers below the blots represent relative expression levels. GM represents growth medium and DM represents differentiation medium.