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Aminoacylation kinetics of AARS protein variants
| Km (mM) | kcat(s−1) | kcat/Km (mM−1s−1) | Ratio to WT | |
|---|---|---|---|---|
| Wild-type | 0.70 ± 0.21 | 0.54 ± 0.04 | 0.81 ± 0.19 | 1 |
| N71Y | 2.9 ± 0.7 | 0.0006 ± 0.0004 | 0.0002 ± 0.0001 | 1/4,130 |
| R329H | 3.0 ± 0.2 | 0.047 ± 0.0001 | 0.016 ± 0.001 | 1/50 |
| E778A | 0.89 ± 0.08 | 0.53 ± 0.001 | 0.60 ± 0.05 | 1/1.4 |
