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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: Plant J. 2011 Oct 10;69(1):14–25. doi: 10.1111/j.1365-313X.2011.04765.x

Figure 4. DRB4 expression in healthy and infected plants.

Figure 4

(a) DRB4 expression pattern in healthy seedlings.

Expression pattern of DRB4-YFP-FLAG fusion protein expressed under the control of the DRB4 native promoter in 4-day old seedlings of two independent transgenic lines (PDRB4:DRB4-YFP-FLAG) was analyzed by epifluorescence microscopy and representative images are shown. C: cotyledon, SAM: shoot apical meristem, H: hypocotyl, R: root, RM: root meristem. Scale bar: 200μm.

(b) DRB4 expression pattern in healthy and systemically infected leaves.

The same lines as in (a) were infected with TYMV. Healthy and systemically infected leaves were observed by epifluorescence microscopy 10 days post-infection and representative images of DRB4 expression patterns are shown. Scale bar: 200μm.

(c) DRB4 transcript accumulation is enhanced in infected plants.

Endogenous DRB4 transcript accumulation was analyzed by RT-qPCR in healthy and systemically infected leaves of WT plants. mRNA levels were normalized to 18S rRNA. The results are presented as the average ± standard deviation calculated from two biological replicates.

(d) DRB4 protein accumulation is enhanced in infected plants.

Total protein content in extracts from healthy and systemically infected leaves of WT and drb4 mutant plants was measured and equal total protein amounts were analyzed by western blot using DRB4-specific antibody. The positions of DRB4 and molecular mass markers are indicated. Ponceau red staining of the large subunit of ribulose-1,5 biphosphate carboxylase (RuBisCo) is shown as a loading control.