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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: Plant J. 2011 Oct 14;69(1):92–103. doi: 10.1111/j.1365-313X.2011.04773.x

Figure 2. Bti9 interacts with AvrPtoB1-307 in an F173-dependent manner.

Figure 2

(A) Interaction in a yeast two-hybrid assay between Bti9 or Pto and AvrPtoB or its variants. A non-specific AvrPtoB tomato-interacting protein, Bti6, encoding a putative lactate dehydrogenase was used as a positive control (Xiao, et al. 2007). Blue patches indicate positive interactions. Photographs were taken 24 hours after yeast transformants were streaked on –Ura –His –Trp plates (Xiao, et al. 2007). (B) Expression of AvrPtoB and its variants as bait proteins in yeast cells that also express Bti9 was confirmed by Western blotting using an anti-LexA antibody.