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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: J Neurochem. 2011 Nov 24;120(1):56–69. doi: 10.1111/j.1471-4159.2011.07559.x

Fig. 1. Partitioning of cellular D2-dopamine receptor (D2R), the mu opioid receptor (MOR) and the delta opioid receptor (DOR) in Triton X-100 (TX100)-soluble and insoluble biochemical fractions.

Fig. 1

D2R (A), DOR (B) and MOR (C) cDNA transfected HEK293T cells and mouse whole brain (Br., excluding cerebellum and brain stem) or mouse striatum (Str.) (D) were solubilized in TX100 detergent-containing buffer. Soluble and insoluble proteins were separated by centrifugation, resolved by SDS PAGE, and the D2R, DOR and MOR proteins in TX100 soluble (S) and insoluble (P, pellet) cell fractions were visualized by immuno-blotting, as described in the Materials and Methods section. CTRL refers to control HEK293T cell samples that were transfected with the corresponding empty plasmid vector. Anti-FLAG refers to the antibody used to visualize the recombinant FLAG epitope-tagged DOR and MOR proteins and anti-D2R refers to the antibody used to visualize D2R. E. Levels of the respective receptor proteins in A, B, C, and D, in TX100-soluble (closed black bars) and insoluble (open white bars) cellular fractions expressed as percentage of the total cellular signal for the respective receptor (mean ± SEM, n=4-8, *represents p<0.01, compared to TX100 insoluble signal for DOR or MOR).