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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: J Neurochem. 2011 Nov 24;120(1):56–69. doi: 10.1111/j.1471-4159.2011.07559.x

Fig. 5. The effect of D2R co-expression on TX100 solubility of mutant RGS9-2 constructs.

Fig. 5

HEK293T cells were transfected with cDNAs for Gβ5 and a DEPless RGS9-2 construct (DEPless) or with cDNAs for Gβ5 and RGS9-2 N364H (N364H). Both RGS9-2 constructs were V5-tagged. Two more experimental groups were transfected in addition, with cDNA for D2R (+D2R). A. RGS9-2 mutant proteins in TX100 detergent soluble (S) and insoluble (P, pellet) cell fractions visualized by immuno-blotting, as described in the Materials and Methods, RGS9-2 N364H (left panels), DEPless RGS9-2 construct (right panels). The distribution of the mutant RGS9-2 constructs in TX100-soluble and insoluble cellular fractions after D2R co-expression (+D2R) is shown in the lower panel. B. Levels of RGS9-2 mutant proteins in TX100-soluble (closed black bars) and insoluble (open bars) cellular fractions visualized in A and expressed as percentage of the respective total cellular mutant RGS9-2 protein (mean ± SEM, n=4-5).