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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: J Neurochem. 2011 Nov 24;120(1):56–69. doi: 10.1111/j.1471-4159.2011.07559.x

Fig.6. Effect of D2R co-expression on the cellular distribution of RGS9-2, RGS9-2 N364H, and Gαi1 in transfected HEK293T cells.

Fig.6

A. D2R distribution in HEK293T cells that were transfected with cDNA for D2R alone. B. RGS9-2 distribution in cells that were transfected with cDNA for the RGS9-2-V5-tag (RGS9) and Gβ5 (Gβ5) (left panel) and in cells that were transfected, in addition, with cDNA for D2R (right panel). C. RGS9-2 N364E distribution in cells that were transfected with cDNA for the RGS9-2 N364E-V5-tag (RGS9 N364E) and Gβ5 (Gβ5) (left panel) and in cells that were transfected in addition with cDNA for D2R (right panel). D. Gαi1 distribution in cells that were transfected with cDNA for Gαi1 alone (left panel) or with cDNA for D2R (right panel). E. Western blot (WB) of Gαi1 in TX100 detergent soluble (S) and insoluble (P, pellet) HEK293T cell fractions visualized by immuno-blotting, as described in the Materials and Methods,