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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: J Neurochem. 2011 Nov 24;120(1):56–69. doi: 10.1111/j.1471-4159.2011.07559.x

Fig. 7. Effect of co-expression of D2R short isoform (D2Rsh) on the TX100 solubility of the RGS9-2/Gβ5 complex.

Fig. 7

HEK293T cells were transfected with cDNA for the D2R short isoform (D2Rsh) alone, or with cDNA for RGS9-2-V5-tagged (RGS9), Gβ5 and D2Rsh. A. D2Rsh protein in TX100 detergent soluble (S) and insoluble (P, pellet) cell fractions visualized by immuno-blotting, using an anti-D2R antibody, as described in the Materials and Methods section. CTRL refers to a control group of cells transfected with empty plasmid vector. B. RGS9-2 (RGS9) protein, co-expressed with D2Rsh, in TX100 detergent soluble (S) and insoluble (P, pellet) cell fractions visualized by immuno-blotting, using an anti-V5 epitope-tag antibody, as described in the Materials and Methods section. C. Levels of D2Rsh and RGS9-2 protein in TX100-soluble (closed black bar) and insoluble (open bar) cellular fractions visualized in B and expressed as percentage of the respective total cellular protein (mean ± SEM, n=3-5).