Table 4.
Prediction of stabilizing/destabilizing and hot spot mutations
| Stabilizing/Destabilizing Mutations | Hot spot Mutations | |||
|---|---|---|---|---|
| Model | Accuracy (%) | Accuracy (%) | Sensitivity (%) | Specificity (%) |
| 1 | 87 | 79 | 54 | 90 |
| 2 | 89 | 82 | 66 | 90 |
Stabilizing mutations had ΔΔGexp < 0 and destabilizing mutations had ΔΔGexp > 0.
Hot spot mutations had |ΔΔGexp|> 2 and non-hot spot mutations had |ΔΔGexp|< 2. Accuracy measures how many ΔΔGcalc have been predicted to be in the same direction of stability as the ΔΔGexp.
Sensitivity measures how many hot spot mutations were identified correctly relative to the total amount of hot spots. Specificity measures how many non-hot spots were correctly identified relative to the total number of non-hot spots. There are 727 destabilizing mutations, 71 stabilizing mutations and 255 hot spot mutations