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. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: Mucosal Immunol. 2011 Sep 21;5(1):19–29. doi: 10.1038/mi.2011.38

FIG. 2. TLR4 mutant mice have an exaggerated PMN influx in response to gonococcal infection.

FIG. 2

Mice were inoculated intravaginally with strain FA1090 or PBS as described in the text, and the vaginal PMN influx was measured by determining the percent of PMNs among 100 vaginal cells in stained vaginal smears. (a,d) PMN count in vaginal smears from infected and uninfected mice. PMN influx in vaginal smears is shown for infected vs. uninfected mice over time for (a) BALB/c and (d) BALB/c-Lpsd mice. Significance was calculated using an unpaired t-test. *, There was a significant difference between infected vs. uninfected strains, with p ≤ 0.05. These results are representative of two independent experiments. (b–c, e–f) Immunohistochemical analysis of PMN influx into the lower genital tract. Genital tract tissue was extracted from infected BALB/c and BALB/c-Lpsd mice mutant mice on day 7 of infection and stained with Gr-1-specific antibodies to detect PMNs, as described in the text. Shown above are representative images for infected (b,c) BALB/c and (e,f) BALB/c-Lpsd mice. Original magnification: 100 × (b, e) and 400X (c, f).