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. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: Mucosal Immunol. 2011 Sep 21;5(1):19–29. doi: 10.1038/mi.2011.38

FIG. 4. Expression of surface TLR2 does not differ between BALB/c and TLR4 mutant mouse strains.

FIG. 4

BMDM were derived from BALB/c and BALB/c-Lpsd mice as described in the text. Cells were stained for surface expression of TLR2 either at rest or after 24 hours treatment with the agents noted above. (a) Shown above are FACS histograms for demonstrating staining using either the specific anti-TLR2 mAb (black line) or a matched isotype control (gray shading). The vertical axis represents the relative cell number, while the horizontal axis represents the intensity of fluorescence in the FL2 channel. The percentage of cells that are either negative or positive for TLR2 is shown for each histogram. (b) The bottom graph shows the median fluorescence as calculated from the histograms for each condition. Treatments were as follows: unstim, unstimulated cells; TNF-α, 40 ng/ml; LPS, 100 ng/ml; H8/Lip, 1 µg/ml; GC lys (N. gonorrhoeae FA1090 crude lysates) MOI 10:1. These histograms are representative of two independent experiments.