A. Enumeration of Salmonella in the cecum of mice at 12 hours following oral infection with indicated Salmonella genotypes. Wild type and avrA− Salmonella were counted following selective propagation on LB agar media plates containing 25μg/ml nalidixic acid, and avrA−/pAvrA was propagated on LB agar media plates containing 25μg/ml nalidixic acid, and ampicillin 100μg/ml, (n=5).
B. Enumeration of Salmonella in the peyer’s patch of mice at 6 h following oral infection with indicated Salmonella genotypes, using selective media as described in 3A, (n=5).
C. Enumeration of Salmonella in the mesenteric lymph node (MLN) of mice 12 h following oral infection with indicated Salmonella genotypes, using selective media as described in 3A, (n=5).
D. Enumeration of Salmonella in the spleen of mice at 24 h following oral infection with indicated Salmonella genotypes, using selective media as described in 3A, (n=5).
E. Body weight loss in mice infected with the indicated Salmonella genotypes, (n=15).
F. Mortality in mice infected with the indicated Salmonella genotypes, (n=5).
G. Spleen weight in mice infected with the indicated Salmonella genotypes for 7 days, (n=5).
H. Serum KC in mice infected with the indicated Salmonella genotypes, (n=5).