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. 2011 Oct 7;44(1-3):97–107. doi: 10.1016/j.molcel.2011.07.034

Figure 3.

Figure 3

Measuring Sister Chromatid Cohesion of Linear Minichromosomes

(A) Schematic representation of the 42 kb linear minichromosome (modified from Murray et al., 1986, with permission from Elsevier).

(B) Differential sedimentation and Southern blot resolution of monomers (dashed orange boxes) and dimers (dashed purple boxes) of linear minichromosomes extracted from wild-type cells (K18251) arrested in metaphase.

(C) Linear minichromosome profiles obtained from G1-arrested cells (K18251) contain exclusively monomeric DNA species.

(D) SCC1's inactivation by cellular growth of scc1-73 strain (K18249) for 2 hr at 37°C in the presence of nocodazole causes the loss of linear minichromosome dimers.