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. 2011 Oct 7;44(1-3):97–107. doi: 10.1016/j.molcel.2011.07.034

Figure 6.

Figure 6

SCI Is Largely Unaffected by Condensin and the Smc5/6 Complex

(A) Wild-type or mutant strains were arrested with α factor pheromone and released into rich media containing nocodazole for 1.5 hr at 37°C. Separation of monomeric and dimeric large circular minichromosomes extracted from wild-type cells (K16150) is shown in (A).

(B) SCC2's inactivation due to growth of scc2-4 strain (K16149) at restrictive temperature causes complete depletion of dimeric minichromosome species.

(C and D) Separation of monomeric and dimeric large circular minichromosomes extracted from smc2-8 (K17893) or smc6-9 (K17895) strains grown at restrictive temperature to induce the inactivation of the SMC2 (C) or SMC6 (D) proteins, respectively.