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. 2011 Oct 7;44(1-3):97–107. doi: 10.1016/j.molcel.2011.07.034

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© 2011 ELL & Excerpta Medica.

Open Access under CC BY 3.0 license

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Cohesin Holds Sister DNAs Together Independent of SCIs

(A and B) Yeast strains (K18072) having Cdc20 under the control of the repressible Met3 promoter (Met3P-Cdc20) and bearing the 26 kb circular minichromosome were arrested with α factor pheromone in −Met-TrpRaff medium and released into YPD + 200 mM methionine with or without nocodazole. Minichromosome cohesion was assessed in the presence (B) or absence (A) of microtubule forces on sister kinetochores.

(C) DNA catenation throughout the cell cycle. During DNA replication (Rep), SCIs arise concurrently with cohesion establishment. Topo II-driven decatenation takes place throughout S and G2 phases, but complete decatenation is hampered by cohesin's embrace of sister DNAs. Following separase-mediated cohesin removal, Topo II resolves the residual DNA catenanes ensuing complete sister separation.