Table 4.
Proteins down-regulated in PLCH compared to non-smoker and/or smoker controls.
| No. of spot | Protein name | AC | Theoretical pI/Mr (KDa) |
Experimental pI/Mr (KDa) |
Mascot search result | Mean %V ± SD × 10-4 | 1-way ANOVA p value | Folds in | Localization | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| No. of matched peptide | Sequence coverage (%) | Score | nsc | sc | PLCH | Nsc-sc | Nsc-PLCH | Sc-PLCH | |||||||
| PLCH < nsc and/or sc | |||||||||||||||
| 25 | Polymeric immunoglobulin receptor | P01833 | 5.58 | 5.14 | 10 | 18 | 129 | 2901±438§ | 3037±1038¥ | 911±694¥§ | 1.31E-03 | 1.04 | 3.18§ | 3.33¥ | Cell membrane |
| 84429 | 87377 | ||||||||||||||
| 26 | Thioredoxin | P10599 | 4.82 | 4.67 | 6 | 40 | 82 | 665±113 | 1492±819¥ | 230±235¥ | 0,005 | 2.24 | 2.89 | 6.48¥ | Cytoplasm-Secreted |
| 12015 | 12098 | ||||||||||||||
| 27 | Plastin-2 | P13796 | 5.2 | 5.19 | 10 | 21 | 102 | 873±355 | 1272±320¥ | 453±386¥ | 0,01 | 1.45 | 1.92 | 2.80¥ | Cytoplasm-Cytoskeleton-Cell junktion |
| 70815 | 61983 | ||||||||||||||
| 28 | Serum albumin | P02768 | 5.92 | 6.04 | 8 | 14 | 92 | 1830±647 | 2980±827¥ | 1239±1363¥ | 0,04 | 1.62 | 1.47 | 2.40¥ | Plasma |
| 71317 | 57513 | ||||||||||||||
| 29 | Serum albumin, fragment | P02768 | 5.92 | 6.09 | 6 | 9 | 74 | 278±137 | 405±224¥ | 124±83¥ | 0,049 | 1.45 | 2.24 | 3.26¥ | Plasma |
| N-term | 71317 | 31059 | |||||||||||||
| 30 | ADP-ribosylation factor-like protein 3 | P36405 | 6.74 | 7.37 | 6 | 52 | 107 | 305±161 | 621±331¥ | 142±49¥ | 0,012 | 2.03 | 2.14 | 4.37¥ | Membrane |
| 20614 | 21404 | ||||||||||||||
| 31 | Alpha-1B-glycoprotein | P04217 | 5.58 | 5.16 | 8 | 22 | 109 | 1370±414§ | 1163±443 | 663±83§ | 0,022 | 1.17 | 2.06§ | 1.75 | Plasma |
| 54809 | 75685 | ||||||||||||||