Figure 2. Ex-FABP tightly binds apo and ferric catecholate siderophores.
Inherent fluorescence was strongly quenched by adding (A) apo-Ent or FeEnt, (B) apo-BB or FeBB, (C) apo-MGE or FeMGE and (D) FePaB to Ex-FABP; derived KD values are listed in Table 1. Point-by-point averages of three independent titrations for each compound are shown with calculated DYNAFIT (Kuzmic, 1996) nonlinear regression fits, used to estimate dissociation constants, superimposed. See also Figure S4.
