Table 1.
Apo and Ferric Siderophore Binding Affinities
| Siderophore | KD (nM)† | Siderophore type | Species |
|---|---|---|---|
| apo(Ent) | 0.5 ± 0.15 | catechol |
Escherichia coli Salmonella enterica |
| [FeIII(Ent)]3− | 0.22 ± 0.06 | catechol |
Escherichia coli Salmonella enterica |
| [FeIII(PaB)]2− | 42 ± 8 | catechol/HPO |
Paracoccus denitrificans |
| apo(BB) | 30 ± 2 | catechol | Bacillus sp. |
| [FeIII(BB)]3− | 14 ± 2.0 | catechol | Bacillus sp. |
| apo(MGE) | 1.1 ± 0.15 | catechol derivative | Salmonella enterica |
| [FeIII(MGE)]3− | 0.07 ± 0.02 | catechol derivative | Salmonella enterica |
| [FeIII(DGE)]3− | >600 | catechol derivative | Salmonella enterica |
| [FeIII(Pyochelin)2]1− | >600 | HPT |
Pseudomonas aeruginosa |
| [FeIII(Pyoverdine)]1− | >600 | catechol derivative |
Pseudomonas aeruginosa |
| [FeIII(Rhizoferrin)] | >600 | citrate | fungal |
| [FeIII(DesferrioxamineB)] | >600 | hydroxamate | Streptomyces pilosus |
| [FeIII2(Alcaligin)3] | >600 | hydroxamate | Bordetella pertussis |
| [FeIII(Petrobactin)]2− | >600 | citrate/catechol |
Bacillus anthracis Bacillus cereus |
| [FeIII(Aerobactin)] | >600 | citrate/hydroxamate | Escherichia coli |
| [FeIII(ExochelinMS)] | >600 | hydroxamate |
Mycobacteria smegmatis |
| [FeIII(Coprogen)] | >600 | hydroxamate | Neurospora crassa |
†
The detection threshold for this assay corresponds to a KD ≤ 600 nM