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. Author manuscript; available in PMC: 2012 Nov 4.
Published in final edited form as: J Diabetes Complications. 2011 Nov 4;25(6):398–404. doi: 10.1016/j.jdiacomp.2011.10.002

Figure 1. Effect of MDI 301 and retinoic acid on MMP-1 activity in organ culture.

Figure 1

Organ culture fluid from untreated, 1 μM and 3 μM MDI 301-treated or 2 μM RA-treated skin was collected on day 4 and assayed for MMP-1 by β–casein zymography. Zymographic images were scanned and digitized, and negative images quantified. Upper Panel: Representative β-casein zymogram demonstrating MMP-1 in organ culture fluid from untreated diabetic skin and skin treated with 1 μM and 3 μM MDI-301 and 2 μM retinoic acid (RA). A higher percentage of MMP-1 in the active form can be seen in culture fluid from untreated skin as compared to MDI 301 and RA treated skin. Lower Panel. Active enzyme expressed as a percentage of total enzyme (densitometry values from active forms divided by values from active + latent forms). Values shown are means and standard errors based on organ cultures from 12 normal and 27 diabetic volunteers. Statistical significance of the differences among the four groups was determined by analysis of variance followed by paired-group comparisons.

*p<0.05 vs untreated samples