A, glucose-stimulated insulin secretion from isolated islets of Kir6.2[AAA]:Cx36+/+ mice (black squares) and Kir6.2[AAA]:Cx36−/− mice (open squares). Data averaged over n = 7 mice. *Significant difference of P < 0.05 (Student's paired t test) at each glucose concentration comparing Kir6.2[AAA]:Cx36+/+ and Kir6.2[AAA]:Cx36−/− data. B, insulin secretion at 2 mm glucose, for intact Kir6.2[WT]:Cx36+/+ (hatched), Kir6.2[AAA]:Cx36+/+ (black), Kir6.2[AAA]:Cx36+/− (grey), and Kir6.2[AAA]:Cx36−/− (white) islets. Data are expressed as a percentage of insulin secretion in Kir6.2[AAA]:Cx36+/+ islets at 20 mm glucose to facilitate comparison with Fig. 4B. ‘ns’ indicates non-significant difference (P > 0.05) comparing each experimental group to Kir6.2[AAA]. C, insulin content of Kir6.2[AAA]:Cx36+/+ and Kir6.2[AAA]:Cx36−/− islets. Data averaged over n = 7 mice. D, glucose-stimulated insulin secretion from dissociated Kir6.2[AAA]:Cx36+/+ (grey triangles) and Kir6.2[AAA]:Cx36−/− (open triangles) β-cells. Displayed is the fractional insulin secretion per hour normalized by insulin content. Data averaged over n = 6 mice. The difference in means ± 95% confidence interval for A and C is shown in Fig. S2C.