Figure 1. Expression of BgKr-h1 mRNA in Blattella germanica determined by qRT-PCR.

(a) Expression in female whole body in the three last nymphal instars: N4, N5 and N6. Relative titers of juvenile hormone III (JH) and 20-hydroxyecdysone (20E) in N5 and N6 are indicated below, according to Treiblmayr et al.²⁸ and Romaña et al.³⁹, respectively. (b) Expression in different tissues of females in day 0 of N6: muscle (M), pronotum (P), mesonotum (Ms), metanotum (Mt), ovaries (O), brain (B), fat body (FB), corpora allata (CA), and in testicles (T) from males of the same age. (c) Effect of the application of 20 µg of JH on freshly emerged N6 on BgKr-h1 mRNA levels. Data in (a) and (c) represent the mean ± SEM, and are indicated as copies of BgKr-h1 mRNA per 1000 copies of BgActin-5c; each point represents 4 biological replicates. Data in (b) represent a pool of 5 specimens. In (c), differences of JH-treated with respect to controls were statistically significant in all cases (p < 0.05), according to the REST software tool⁴⁰.