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2 µg of untagged WhiA was incubated with 2 µcro;g h6-WhiG, purified with Ni-NTA resin, washed 5 times and separated on SDS PAGE followed by silver staining. Lanes 4 and 5 contain 1/20 the amount of protein used in the pull-down reactions. An “*” indicates a breakdown product of WhiA that corresponds to the LAGLIDADG domain.
