Table 3. Properties of known GpgPs and one MpgP (with GpgP activity). The M. tuberculosis GpgP belongs to the histidine phosphatase superfamily (His-Phos) while the other proteins belong to the HAD-like hydrolase superfamily.
| GpgP activity | Optimum pH | aOptimum temperature | Km GPG (mM)b | Vmax (µmol/min.mg)b | Cation dependence | Reference |
|---|---|---|---|---|---|---|
| Mycobacterium tuberculosis (His-Phos) | 7.0 | 45°C | 0.35 | 67.2 | Independent | This study |
| Methanococcoides burtonii (HAD-like) | 6.0 | 50°C | 0.08 | 0.10 | Co2+>Mg2+>Ni2+ | 20 |
| Persephonella marina (HAD-like) | 7.0 | 85°C | 0.36 | 97.0 | Mg2+>Co2+>Mn2+ | 27 |
| Thermus thermophilus (HAD-like) | 6.0 | 95°C | 0.83 | 46.7 | Co2+>Mg2+>Mn2+ | 20, 28 |
aTemperatures at which GpgPs show maximal activity.
bKm and Vmax values were determined at each organism's optimum growth temperature (M. tuberculosis, 37°C; M. burtonii, 30°C; P. marina, 70°C; T. thermophilus, 70°C).