Figure 5:

Activity of matrix metalloproteinase-9 determined by gelatin in vitro zymography. Values represent MMP-9 semi-quantitatively. Activities between groups differed significantly (P = .0003). Control and sham did not differ statistically significant (P > .05). Differences between control and IR30/60 reached a statistically significant level (P < .001). Differences between sham and IR30/60 showed a trend toward significance (P > .05). GSH preconditioning (GSH-IR30/60) effectively reduced MMP-9-activity during IR and showed to trend toward statistical significance when compared to IR30/60 (P > .05). Values of IR-IR30/60 almost equaled values of sham (P > .05). Data are expressed means ± SEM.