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High-density culture of Y. lipolytica producing huIFN α2b. During induction, oleic acid was added continuously to the bioreactor at a flow rate of 1.25 g h-1; pepstatin was added, at a concentration of 10 μM, at the start of induction. Time course of biomass (open circle), huIFN α2b production (closed circle) and protease activity (open triangle) (A). Western blot analysis showing huIFN α2b as a single full-length form (B).
