Table 1.
Comparison of kinetic parameters of huIFN α2b production under different induction strategies
| Parameter | Strategy | |||
|---|---|---|---|---|
| 1 | 2 | 3 | 4 | |
| St (g L-1) | 20 | 10 | 20 | 20 |
| Si (g L-1) | 20 | 10 | 5 | a |
| Induction period (h) | 55 | 54 | 33 | 21 |
| Sc (g L-1) | 11.14 | 6.3 | 13.8 | 17.3 |
| X (g L-1) | 84.7 | 92.3 | 83.7 | 65 |
| YX/S (g g-1) | 0.96 | 3.06 | 0.77 | 0 |
| huIFNα2b (mg L-1)b | 185 | 207 | 280 | 355 |
| V (mg L-1 h-1) | 3.36 | 3.83 | 8.5 | 16.9 |
| P (mg g-1 h-1) | 0.04 | 0.041 | 0.1 | 0.26 |
a Oleic acid was add by a continuous feeding, at a rate of 1.25 g h-1
b Mean level of huIFNα2b
St: total oleic acid concentration, Si: initial oleic acid concentration, Sc: substrate consumed. X: mean biomass level during induction, V: mean volumetric production of huIFN α2b, P: mean specific production of huIFN α2b